Ba/F3 transformation assays

Ba/F3 transformation assays

ECVAM prevalidation of three cell transformation assays.

A prevalidation study on the cell transformation assays in SHE cells at pH 6.7, SHE cells at pH 7.0 and Balb/c 3T3 cell line was coordinated by ECVAM focussing on issues of standardisation of protocols, within-laboratory reproducibility, test method transferability and between-laboratory reproducibility. The Validation Management Team concluded that standardised protocols are now available that...

Unwanted acquired mutations in Ba/F3 transformation assays

Cancer genome sequencing has unraveled the huge variety of DNA alterations acquired during cell transformation. Only a small part of these alterations are thought to promote cancer development, while others represent mere random passenger mutations. It is essential to decipher which mutations are driving the oncogenic process to translate cancer sequencing data into clinically meaningful inform...

Genetic transformation assays for identification of strains of Moraxella urethralis.

Studies of 31 strains of Moraxella urethralis have shown that 20 of them are competent for genetic transformation. This finding has led to the development of transformation assays for identification of newly isolated strains of this organism. Crude deoxyribonucleic acid (DNA) samples from all strains of M. urethralis readily transform auxotrophic mutants of competent strains to prototrophy, whe...

Cell transformation assays as predictors of human carcinogenicity.

4FRAME, Russell & Burch House, 96–98 North Sherwood Street, Nottingham NG1 4EE, UK; 5ECVAM, JRC Institute for Health and Consumer Protection, 21020 Ispra (VA), Italy; 6Institute for In Vitro Sciences, 21 Firstfield Road, Suite 220, Gaithersburg, MD 20878, USA; 728/30 Via Trieste, 21030 Brinzio (VA), Italy; 8Division of Carcinogenesis and Differentiation, FSII, 0240, German Cancer Research Centr...